Unlike the tins and pin collections, the promo cards that come in ETBs are typically plastic. For 49.99, the Shining Fates ETB comes with ten packs, 60 sleeves, play materials like counters, tokens, and energy cards, and an Eevee VMax promo card. If you’re going up against a Lightning-type deck, you can use Mewtwo & Mew- GX’s Perfection Ability to copy the attacks of Moltres & Zapdos & Articuno- GX and keep the TAG TEAM trio safe from Lightning-type Pokémon that can hit it for double damage. As with any set, the real value is in the Elite Trainer Box. Of course, once you've powered up to use Trinity Burn, you also have enough Energy for the trio’s Sky Legends- GX attack, which hits three of your opponent’s Pokémon for 110 damage and then shuffles the TAG TEAM into your deck, preventing your opponent from getting a 3-Prize Knock Out. After doing this, you just need a Fire Energy and one other Energy of any type to begin using the trio’s Trinity Burn attack, which hits for an impressive 210 damage. Official Pokmon TCG: Hidden Fates Elite Trainer Box with 10 booster packs, a full art foil promo card featuring Moltres & Zapdos. A brand-new, unused, unopened, undamaged item. You can then use Dragonite’s Hurricane Charge Ability to help attach Water and Lightning Energy to the TAG TEAM trio. Pokemon Hidden Fates Elite Trainer Box ETB AUS In-Stock Sealed FAST SHIPPING Condition. To assist Moltres & Zapdos & Articuno- GX, you’ll want to get Dragonite out as soon as possible. Viridian Forest and Energy Spinner both let you search your deck for any type of basic Energy, so they will prove invaluable to get the proper Energy into your hand. SKU: SUN&MOONLAUNCHBUNDLE Categories: Hidden Fates, Sun & Moon. Out of Stock Sun & Moon Launch Box + 2x Hidden Fates ETBs 499.99. Today we're opening the CHEAPEST Pokemon Hidden Fates Elite Trainer Box I've ever seen I bought this Hidden Fates ETB for super cheap and. The first thing to keep in mind is that Moltres & Zapdos & Articuno- GX's attacks require three different types of Energy to use (Fire, Water, and Lightning). Home Shop Sun & Moon Launch Box + 2x Hidden Fates ETBs. Once you get it there, though, this card can do some major damage to your opponent’s Pokémon. Hidden Fates is a special expansion of the Sun & Moon Series of the Pokémon Trading Card Game.In Japan, it was released as GX Ultra Shiny, the twelfth subset in the Pokémon Card Game Sun & Moon Era.It was the second to be considered as an enhancement of the eighth Sun & Moon Era main expansion, Super-Burst Impact. Moltres & Zapdos & Articuno- GX is a powerful card, but it requires a bit of effort to power it up to its full potential. The Pokémon TCG: Hidden Fates expansion features the first TAG TEAM trio, and this deck is designed to take advantage of the power of that card.
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The album was titled Time Out, and its singles ‘Take Five’ and ‘Blue Rondo a la Turk’ were the first of modern jazz. In 1960, the Dave Brubeck Quartet, along with Paul Desmond, Eugene Wright, and Joe Morello, released their first experiment in odd-meter rhythms. In 1954, his photograph appeared on the cover of Time magazine, associated with the history of the jazz revival. In the bop era, Dave Brubeck’s quartet managed to be number one on the jazz charts of a black newspaper, The Pittsburgh Courier. They played in top-tier jazz clubs, as well as touring with Charlie Parker, Dizzy Gillespie, Stan Getz, and other great jazz musicians. It was they who rode the wave of jazz popularity on college campuses during the 1950s. The Dave Brubeck Quartet became the sound that identified an era. His group managed to reach number one on Down Beat magazine’s Critics and Readers lists. His daring improvisations and his harmonic approach generated great interest from critics and fans alike. The first impression of the public associates it with an intricate and light sound known as West Coast Cool. Brubeck was a very prolific and original songwriter and some of his best known tunes include The Duke, In Your Own Sweet Way and Blue Rondo a la Turk.ĭave Brubeck became a jazz legend. In the 1970s, Brubeck toured and recorded several times, using his own children as staff. But despite the great general success with the public, Brubeck failed to gain deep and complete acceptance as a piano teacher in the jazz environment. This quartet, which featured saxophonist Paul Desmond, flirted during the 1960s with metrics then foreign to jazz, and became in one of the most famous groups within this genre. In 1946, Brubeck constituted an experimental octet he then created a trio in 1949 and finally a quartet, with whom he performed between 19. He went on to serve in the United States Army in World War II and later returned to music, resuming his studies under the watch of Arnold Schoenberg, who had invented the twelve-tone system of composition. He studied composition at Mills College in Oakland, California, under the tutelage of French composer Darius Milhaud. He worked as a pianist with local jazz groups from 1933 and studied music at the College of the Pacific between 19 in Fresno, California, where he later created and conducted his own twelve-piece orchestra. Brubeck learned piano as a child from his mother, Elizabeth, who was a piano teacher and taught him since he was four years old At the age of nine, he also began to practice on the cello. He is known for incorporating elements of classical music into jazz. American composer and pianist born on December 6, 1920, in Concordia (California) and died on December 5, 2012, in Norwalk (Connecticut). His marriage to the former Margarette Torbert ended in divorce. He was a member of the Kenwood Golf & Country Club. His honors included an honorary doctor of laws degree from Amherst. He retired in 1979 and became a consultant. Ballantine returned to the United States in 1962 and joined the staff of the World Bank, where he was director of finance for educational projects. Robert College is now called the University of the Bosphorus. He also arranged for control of the schools to be transferred from their American trustees to the Turkish government. He then served as president of Reed College until 1955, when he went to Istanbul to head Robert College and the American College for Girls.ĭuring his years in Turkey, he worked to have the two schools merged. Ballantine taught history at the Massachusetts Institute of Technology from 1945 to 1952. Naval Logistics in the Second World War." After the war, his doctoral thesis was published under the title, "U.S. He graduated from Amherst College and received a doctorate in history from Harvard University.ĭuring World War II, he served in the Navy. But he didn't like the name and let only a few friends address him that way.ĭuncan Smith Ballantine, 81, a retired education finance official at the World Bank and a former president of Reed College in Portland, Ore., and Robert College in Istanbul, died of heart ailments Dec. He got his nickname from Humphrey Bogart in 1955 when he arranged five roles for the actor in just one day. He became an agent when he learned the cut was 10 percent. He began his show business career as a lawyer for the MCA talent agency in the 1930s. Lazar to cancel his bash because it was drawing bigger celebrities than the show itself. In 1975, the producer of the Academy Awards asked Mr. He also was known for the star-studded parties he threw annually to mark the Academy Awards. "I was in my early thirties, and he was right," Dickinson confided to People magazine in 1989. Lazar when he told her she was too old to wear hot pants. Preminger needed 50 stitches near one eye after Lazar hit him with a water glass.Īngie Dickinson, however, didn't clout Mr. He made headlines in 1965 after a scuffle with director Otto Preminger at New York's 21 Club. Nixon, Noel Coward, Tennessee Williams, Irwin Shaw, John Cheever, Art Buchwald, Ira Gershwin, Moss Hart, Lillian Hellman, John Huston, Alan Jay Lerner, Richard Rodgers, Herman Wouk, Bob Woodward and longtime friend Ronald Reagan.Īnother of his clients, Quentin Reynolds, the writer and commentator, gave the agent a silver box inscribed, "Lazar is our shepherd, we shall not want." Over the years, his clients included Cole Porter, Ernest Hemingway, Faye Dunaway, Vladimir Nabokov, Richard M. He sold Neil Simon's play "The Odd Couple" when it was only a five-sentence summary, a 1966 Newsweek article said. In the 1960s, when such mega-deals were unheard of, he sold Truman Capote's novel "In Cold Blood" for $500,000 and the musical "Camelot" for $1.4 million. Lazar, who wore trademark oversize dark eyeglasses, called himself the Prince of Pitch, and he won record fees for his clients with his coaxing, coddling and bluster. Irving "Swifty" Lazar, 86, the legendary Hollywood talent agent whose image as a dapper, elfin dealmaker with Coke-bottle glasses and bald dome made him a caricaturist's dream, died of kidney failure Dec. Wigglesworth of Wheaton, and misspelled the name of a daughter, Mary Patricia Fissell. It also gave an incorrect name and town of residence for another son, Arthur M. Wigglesworth of Arcadia, Calif., from the list of survivors. Wigglesworth, which appeared yesterday, omitted a surviving son, Pierre G. By default, ddclient is setup to use SSL, as shown below. The configuration file is located at /etc/nf, but there is a directory at /etc/ddclient with a configuration file for each interface. interface : eth0Īlso, just so you know, EdgeOS uses ddclient for the dynamic DNS updates. You can show the status with the command below. It’s not the best fit for the ERL, so I don’t recommend getting that. That didn’t work, so I tried replacing the flash drive with a 4GB Sandisk Cruzer Blade. Trigger a manual update. EdgeOS will only update the dynamic DNS provider when your IP address actually changes. In my first attempt at recovering the EdgeRouter Lite, I used Ubiquiti’s official guide found here. the password is your updater client key (that long string of numbers/letters).the hostname is the entire domain (e.g., ).Set service dns dynamic interface eth0 service dyndns server Set service dns dynamic interface eth0 service dyndns protocol dyndns2 Set service dns dynamic interface eth0 service dyndns password updater-client-key Set service dns dynamic interface eth0 service dyndns login username Set service dns dynamic interface eth0 service dyndns host-name set service dns dynamic interface eth0 service dyndns set service dns dynamic interface eth0 service Router setup #ĮdgeOS only supports a handful of pre-configured DNS service providers by default (shown below). Go to your Account Settings page and make note of the Updater Client Key. For this setup, just click Your current location’s IP address to continue through to this process. Create a new hostname, choosing the TLD from the available options. Head over to the Dyn website and setup an account. They now charge $40/year for 30 hostnames. I should mention, however, that Dyn no longer offers free dynamic DNS (it was discontinued in 2014). In the past, I’ve posted about my DuckDNS dynamic DNS settings, and mentioned I use Dyn as well. Ubiquiti UniFi controller setup on Raspberry Pi 3 I have it all set up in OpenDNS the way I want it, but when I go to System>Name Servers and add the DNS servers (208.67.222.222) and (208.67.220.220), apply the settings, then go to to see if it works, the site reports that it isn't working. Ubiquiti EdgeRouter serial console settings Hello all, I am trying to get OpenDNS to work on my EdgeRouter Lite but have run into a problem. Migrating away from the Ubiquiti EdgeRouter Lite I use the second method as it keeps the DNS forwarding options in one section of the config file instead of two.Hey! Listen! This post is part of a series on the Ubiquiti EdgeRouter Lite. Explicitly configure DNSmasq forwarders using set service dns forwarding options server= DNS_Server_IP.The local system will always attempt resolution starting with the first entry in /etc/nf, while DNSmasq will ignore 127.0.0.1 and use the additional entries as forwarders. Specify addition nameservers for this option.This step assumes that you haven’t done so. I performed all of the steps here under macOS, so you may have to modify depending on your computer’s OS. To configure the upstream forwarders there are two options: The recovery instructions covered here contains some modifications from the original post. The way to minimize the number of local DNS lookups that get forwarded is to specify 127.0.0.1 as the system nameserver, so they go through DNSmasq and are routed and cached the same as DNS requests from clients. If your upstream DNS provides any DNS reporting the DNS lookups for those services will be at the top of your reports. In each of these instances, DNS lookups are performed by the system at intervals of 1 minute or less. Multi-WAN connectivity tests if you have not explicitly set an IP-based test target.There are several situations where this does matter: The system itself, however, will use those entries directly for DNS resolution - bypassing DNSmasq and not performing any caching.įor many deployments this doesn’t matter - a typical configuration won’t have the router itself initiating many DNS lookups. DNSmasq will pick up those entries as forwarders. Whatever is entered here gets put at the top of /etc/nf. There’s a fair amount of EdgeRouter guidance suggesting the System Name Server be configured, primarily in conjunction with set interfaces ethernet eth X dhcp-options name-server no-update in order to specify nameservers other than what the upstream ISP provides. “We think of melancholy today as something that needs to be corrected through pharmaceuticals and talk therapy, but back in the 18th century it was something that people actually sought out, that idea of melancholia.” “It was the picturesque movement and sublimity, the experience of melancholy, which ruins were a part of … creating that kind of sublime moment,” Dodds said. The artist lived in the age of melancholia, where ruins were romanticized and often the subject of artistic works, as Roman artists set out to prove that Rome was the artistic center of the world, rather than Greece. I suspect his home life was less than lovely.” Like any genius who creates a world unto themselves, there’s a lot of wreckage from that. “He was a little crazy - some would say a lot crazy, and he was a genius, and he created a world unto himself. A prolific creator, he produced more than 1,028 copper plates in his short lifetime, as well as sold prints, either as singular copies or bound in books. Piranesi, who lived from 1720 to 1778, owned a print shop below his home, where he engraved incredibly detailed drawings on copper plates, a medium which does not easily lend itself to the intricacy that Piranesi mastered. 20, aims to teach architecture students about innovative architectural drawing through the work of Piranesi, an 18th century Italian architect, artist and theorist whose work redefining perspective forever solidified him in architectural fame.Īlvin and Sally Beaman Professor of Architecture George Dodds, who has been teaching at UTK since 2000, curated the exhibit, and all but one of the Piranesi works featured hail from Dodds’ personal collection. “Learning from Piranesi: Architectural Representation and Tectonics: An Exhibition Celebrating the 300th Birth Anniversary of Giovanni Battista Piranesi,” an exhibit opening at the Ewing Gallery at UTK on Jan. Today, Giovanni Battista Piranesi continues to influence architectural work - and may even play a crucial role in reckoning with modern changes in architectural understanding. Nearly 300 years ago, one Veneto-born architect radically transformed interpretation of ruins, melancholia and architecture. Ralph Lauren opened its first shop in New York in 1969. Polo Ralph Lauren Brand Outlet Leeds LS2 7AU (+44 )1132457424 Ralph Lauren Polo Brand Outlet Liverpool L1 3DE (+44 )1513191488 Contact Info. Buy hand-picked Ralph Lauren products at the best prices. This offer can only be combined with applicable offers for free shipping. POLO RALPH LAUREN Slim-fit basic mesh cotton piqu polo shirt with Pony Player. Discover Ralph Lauren fashion outlets in the UK with our directory of stores. 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Shop designer clothing online on Ralph Lauren MYs official store including the Polo Ralph Lauren collection. THIS OFFER IS NOT VALID AT POLO RALPH LAUREN FACTORY STORES. PT on October 10, 2022, and is valid for three (3) uses only on select full-price and sale styles as marked at, The Ralph Lauren App, and select full-price Ralph Lauren stores. Ralph Lauren reserves the right to end or modify any free shipping offers at any time.Ĭreate Custom Gifts for Father’s Day: Order by 6/12 at 11:59 PM ET | Shop Now This offer is not applicable to purchases being shipped internationally. Any philanthropic donations added at checkout do not count toward the minimum purchase amount. 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Much like his early idol, Frank Zappa, John is a fan of shaping sound with effects like echo, reverse, and modulation (phasing, flanging, chorus, etc.). Even more remarkable is that he’s not afraid to take chances and be experimental, whether with his playing style or his use of electronic effects. With most guitarists, this sometimes affects their ability to play with looseness because they are trying so hard to be perfect, but this isn’t the case with John.Īlthough, he can demonstrate his virtuosity with the instrument, he deliberately downplays this because he feels that his music needs to be more organic and natural. He is noted for his knowledge of music theory and perfectionism. John’s technique could best be described as melodically driven allowing the melody of the tune to dictate his playing. Tracks like Hump de Bump and Storm in a Teacup recall some of the earlier Chili Peppers albums with their funk, rap and R&B influences. Stadium Arcadium was in some ways a progression from By the Way, but in other ways, it showed that John was more comfortable in being able to look back and reincorporate a funk sound into some of their music. The song On Mercury featured elements of ska and the heavily flamenco influenced Cabron showed that the band, and by extension, Frusciante, were willing to take some real chances. Nearly absent of singer Anthony Kiedis’ rap/rock lyrical style, we also find John branching out in more acoustic and melodically complex directions. 2002’s By the Way was much more pop oriented than previous albums. John’s third and fourth albums with the band further solidified and refined his sound. This was apparent not just on more mellow numbers such as Scar Tissue and Otherside, but also on more driving songs like Around the World. It’s impossible to determine what it was that changed in the period from 1993-1998, since, by his own admission, much of it is lost to a drug induced haze but upon his return, John’s sound maintained much of its earlier funk flavor, but it was now more mature and refined, having lost some of its former looseness. He ended up conquering his demons and returned to the band in 1998 following the departure of Dave Navarro. He also was dealing with personal issues, including growing substance abuse issues. John left the band in 1993, deciding that the band had become “too popular” leaving him unhappy. The jazz-flavored Under the Bridge shows John becoming comfortable with a more subdued and understated tone while still featuring the guitar as a key element in the mix. The quirky Mellowship Slinky in B Major harkens back to the Frank Zappa influences in his past with its complex fret work and studio tape effects. The first single off the album, the frenetic Give it Away, shows him really solidifying the funky, sometimes staccato groove that would become instantly identifiable as his unique tone. For me, striving for the perfection he was known for demanding from his bands was a powerful motivation and force behind the huge amount of practicing I was doing back then.” In the liner notes for the 2007 Frank Zappa album The Dub Room Special!, Frusciante writes, “By the time I was 15 I owned all of the records and was spending about 70% of my musical life studying and learning his music. His earliest influences were not that different from any of the thousands of other young guitarists of the day, basically, Jeff Beck, Jimmy Page, David Gilmour and Jimi Hendrix.Īt age 11, he discovered Frank Zappa, and by age 15, it had become an obsession. He was an unusual choice for the band since he did not have a background in funk music as the previous guitarists had. John was just 18 when joined the Chili Peppers in 1988 following the firing of Blackbyrd McKnight, who himself was a recent replacement for original guitarist Hillel Slovak who died of a heroin overdose earlier that year. We’ll take a look at John Frusciante’s gear during the Chili Peppers years to analyze how he achieved his tone, and look at some of the artists that inspired him and how their playing style and gear influenced him. Part of that can be attributed to his playing style, while the rest can be put down to the guitars, amps and effects he used. Massive hits like Give It Away, Under the Bridge and Otherside sound nothing like anything that came before them. John Frusciante’s name might not be immediately recognizable to the average music fan, but as lead guitarist for the funk rock group Red Hot Chili Peppers from 1988-1992, and again from 1998-2009, his tone is very distinct and instantly recognizable. SMITHING Blast furnace: up to 900K Profit/hr 2 2. To maximise profit, use the following items to maximise the crop yield: Magic secateurs from Fairy Tale I - Growing Pains, which boosts yield 10. Here are the top 10 P2P skilling money makers in OSRS, ranked in order by requirements (easiest > hardest). Watch 01:33 It's a Me, Mario - The Loop See also: Calculator:Farming/Herb profits Farming grimy lantadyme can be a very profitable way to spend a few minutes at a time. It can be taken from the table in the Captured Temple by hand only if the six nearby Monk of Zamorak§Monks of Zamorak are dead, or by using Telekinetic Grab, which requires 33 Magic. Even though we recommend PVM for moneymaking, there are many skills in osrs that can make you decent money and experience. Cadantine is the only high-level herb with a decent profit margin, although the gold per hour is very similar to growing Irit leaves. §Details = File:Captured Temple map.png§right§200px Wine of Zamorak is used to create super ranging potions and super Zamorak brew. Note: All prices are calculated using current Grand Exchange market prices, meaning the actual profit per hour may vary. If you have a correction for a guide or have a suggestion for a new method, please leave a message on the main talk page. Please note that grimy avantoes have a buying limit of 10,000 every four hours, so you may clean them faster than you can buy them.Ĭoin profits guide/Cleaning grimy avantoe=Coin profits guide/Cleaning grimy avantoe|=|=|%ARGS%=§Activity = Cleaning Grimy avantoe For a full list of all the money making methods, see Money making guide. It can take a good amount of time to fill up your inventory, depending on your level and Pickaxe, but since each Ore is 3.6k, you will get around 300k per hour. Due to the risk of repetitive strain, it is advised to use make-x to speed up the cleaning process. Like all methods involving large amounts of buying and selling, it is recommended to test the market first to make sure cleaning avantoe is profitable.Ģ8 herbs can be cleaned per inventory, and each inventory can take from 10 to 20 seconds. And that’s it These are my absolute best tips and methods for making money in RuneScape, both Old School and RS3. It is often profitable to clean the grimy herbs and sell them on the Grand Exchange. It can take a good amount of time to fill up your inventory, depending on your level and Pickaxe, but since each Ore is 3.6k, you will get around 300k per hour. Download EpicBot: OSRS Bot, Old School RuneScape. Location = Grand Exchange or bank chest recommendedĭetails = Avantoe are in high demand to create Super energy and Extreme attacks. It is also going to ban your gold farm profile, if they think that suspicious activity is occurring on it. eager: Default loading behavior of the browser, which is the same as not including the attribute and means the image is loaded regardless of where it's located on the page.lazy: Defer loading of the resource until it reaches a calculated distance from the viewport.Here are the supported values for the loading attribute: You can use the loading attribute to completely defer the loading of offscreen images that are reached by scrolling: Images below the viewport are loaded with a lower priority, but they're still fetched as the page loads. The loading attribute #Ĭhrome loads images at different priorities depending on where they're located with respect to the device viewport. Browser-level lazymloading also ensures that deferred loading of images still works even if JavaScript is disabled on the client. With lazy loading supported directly by the browser, however, there's no need for an external library. Using scroll, resize, or orientationchange event handlersĮither option can let developers include lazy loading functionality, and many developers have built third-party libraries to provide abstractions that are even easier to use.Previously, there were two ways to defer the loading of off-screen images: At the 90th percentile, sites send over 5 MB of images on desktop and mobile. Why browser-level lazy loading? #Īccording to the HTTP Archive, images are the most requested asset type for most websites and usually take up more bandwidth than any other resource. Browser compatibility #īrowsers that do not support the loading attribute simply ignore it without side effects. You can use the loading attribute to lazy-load images without the need to write custom lazy loading code or use a separate JavaScript library. All articles Browser-level image lazy loading for the webīrowser-level support for lazy loading images is now supported on the web! This video shows a demo of the feature:.Does Lighthouse recognize browser-level lazy loading?.How are images handled when a web page is printed?.How does browser-level lazy loading affect advertisements on a web page?.Is lazy loading for iframes also supported in browsers?.How do I handle browsers that don't support lazy loading?.What if I'm already using a third-party library or a script to lazy-load images?.How does the loading attribute work with images that are in the viewport but not immediately visible (for example: behind a carousel, or hidden by CSS for certain screen sizes)?.Is there a downside to lazy loading images that are within the device viewport?.Can CSS background images take advantage of the loading attribute?.Can I change how close an image needs to be before a load is triggered?.Are there plans to automatically lazy-load images in Chrome?.Avoid lazy loading images that are in the first visible viewport.Images should include dimension attributes.Improved data-savings and distance-from-viewport thresholds.Relationship between the loading attribute and fetch priority. The current standard of care within the United Kingdom for locally advanced rectal cancer is long course chemoradiotherapy, however the recent development of the concept of “total” neo-adjuvant therapy (TNT), whereby consolidation chemotherapy is given after chemoradiotherapy, increased pCR rates from 15 to 25% in the consolidation group, acting as an additional therapeutic option in these patients which may lead to its introduction as standard of care. Multiple investigators have shown higher rates of response with higher radiotherapy doses, with maximum pCR rates of 25–30%. The former regimen usually demonstrates little, if any tumour regression, but if it does occur, is associated with more favourable prognosis, and the latter regimen can lead to significant tumour shrinkage and downstaging, with pathological complete response (pathCR, defined as complete regression of tumour in the resection specimen) observed in approximately 10–15% of patients. Treatment typically consists of excisional surgery with neoadjuvant therapy if the cancer is locally advanced, consisting of either short course radiotherapy (25 Gy in 5 fractions over 1 week) with surgery the following week, or long course neo-adjuvant chemoradiotherapy (nCRT) (45–50 Gy in 25 fractions over 5 weeks, with synchronous iv 5-FU or oral capecitabine) with surgery 6–10 weeks later. Rectal cancer is a common malignancy, with approximately 11,000 cases per year in the UK. Potential therapy resistance mechanisms involve the PI3K/AKT/mTOR signalling pathway, but tumour heterogeneity does not seem to play a role in resistance. The phenomenon of pathCR in rectal cancer may be related to immunovisibility caused by a high tumour mutational burden phenotype. Methylation analysis demonstrated enrichment for hypomethyation in the PI3K/AKT/mTOR signalling pathway. There were no significant differences in tumour heterogeneity as measured by MATH score. Patients in the pathCR group had significantly higher tumour mutational burden and neoantigen load, frequent copy number alterations but fewer structural variants and enrichment for driver mutations in the PI3K/AKT/mTOR signalling pathway. Twenty four patients who underwent pathCR and twenty-four who underwent poor response underwent molecular characterisation. Pre-treatment biopsies of cancers from these patients underwent high read depth amplicon sequencing for a targeted panel, exome sequencing, methylation profiling and immunohistochemistry for DNA repair pathway proteins. Two groups of patients with either complete response (pathCR group, N = 24) or no response (poor response group, N = 24) were retrieved. We hypothesised that the changes seen in the pre-treatment biopsies of pathCR but not seen in residual tumour after chemoradiotherapy were the determinants of responsiveness. Pathological complete response (pathCR) in rectal cancer is beneficial, as up to 75% of patients do not experience regrowth of the primary tumour, but it is poorly understood. |